Histological Analysis of TA and DIA Muscles

The TA and DIA muscles were sectioned at 8 μm using a CryoStar NX70 cryostat (Thermo Fisher) and stained with H&E or Sirius red. IgG staining was performed to detect degenerating fibers as previously described (54 (link)). In brief, the cryosections were preincubated with 5% BSA in PBS for 1 h, followed by incubation with Alexa 488-conjugated goat anti-mouse IgG antibody (A-11029, Invitrogen, Carlsbad, CA). Fluorescence images were obtained using a BZ-X810 fluorescence microscope (Keyence).

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Nogami K., Maruyama Y., Sakai-Takemura F., Motohashi N., Elhussieny A., Imamura M., Miyashita S., Ogawa M., Noguchi S., Tamura Y., Kira J.I., Aoki Y., Takeda S, & Miyagoe-Suzuki Y. (2021). Pharmacological activation of SERCA ameliorates dystrophic phenotypes in dystrophin-deficient mdx mice. Human Molecular Genetics, 30(11), 1006-1019.

Publication 2021

CryoStar NX70 cryostat Alexa 488-conjugated goat anti-mouse IgG antibody BZ-X810 fluorescence microscope

Anti igg Antibody Cryosections Fluorescence Fluorescence microscope Goat Mouse Muscles

Corresponding Organization : National Center of Neurology and Psychiatry

Other organizations : Kyushu University, Tokyo University of Science, Minia University, Nippon Sport Science University

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Variable analysis

independent variables

Sectioning of the TA and DIA muscles at 8 μm using a CryoStar NX70 cryostat

dependent variables

Staining with H&E or Sirius red
IgG staining to detect degenerating fibers

control variables

Preincubation of cryosections with 5% BSA in PBS for 1 h
Incubation with Alexa 488-conjugated goat anti-mouse IgG antibody

controls

Positive control: Not specified
Negative control: Not specified

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