Two strains of primary human small airway epithelial cells (hSAEC) from different donors were purchased from Lonza Inc., (Allendale, NJ) and cultured in small airway epithelial cell growth medium (Lonza) supplemented as recommended by the supplier, and used for experiments at early passage (16 (link)). For the preparation of SAECs containing an NF-κB-luciferase reporter, hSAECs were infected with commercially available ready-to-transduce lentiviral particles that express the firefly luciferase gene under the control of a minimal CMV promoter and tandem repeats of the NF-κB transcriptional response element (SA Biosciences; Valencia CA). Typically, hSAECs were pre-incubated for 24 hours in basal medium (without supplements) to minimize the effect of medium components on inducing inflammatory signaling. Cells were then pretreated with RvD1 for 30 minutes, followed by 5μ/ml of high molecular weight (HMW) poly(I:C) (Invivogen, San Diego, CA) for the indicated time points.