VSMC were transfected using Lipofectamine LTXTM and Plus Reagent (Invitrogen). cIAP2 constructs were co-transfected together with pCMV5/NOR-1 expression plasmid or the corresponding empty vector (pCMV5)52 (link). Luciferase activity was determined in cell lysates using the Dual-LuciferaseTM Reporter Assay System (Promega). Results were expressed as the ratio of firefly to renilla activity.
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