CXCR4-Mediated Calcium Signaling

Calcium mobilization was examined using flow cytometry as described.^{23 (link)} Cells were stimulated with 200 ng/mL of recombinant human/rhesus macaque/feline CXCL12/SDF-1 alpha (R&D Systems, Minneapolis, MN, USA) in the presence or absence of CXCR4 blockade using 500 nM of CXCR4 antagonist AMD3100 (Sigma, Saint Louis, MO, USA) for 15 minutes on ice. Ionomycin was used as a positive control for calcium mobilization in each cell line.

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Im K., Graef A.J., Breen M., Lindblad-Toh K., Modiano J.F, & Kim J.H. (2015). Interactions between CXCR4 and CXCL12 promote cell migration and invasion of canine hemangiosarcoma. Veterinary and comparative oncology, 15(2), 315-327.

Publication 2015

recombinant human/rhesus macaque/feline CXCL12/SDF-1 alpha AMD3100

Amd3100 Calcium Cell line Cells Cxcr4 Feline Flow cytometry Human Ionomycin Rhesus macaque Sdf 1

Corresponding Organization : Uppsala University

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Variable analysis

independent variables

Presence or absence of CXCR4 blockade using 500 nM of CXCR4 antagonist AMD3100

dependent variables

Calcium mobilization

control variables

Cell stimulation with 200 ng/mL of recombinant human/rhesus macaque/feline CXCL12/SDF-1 alpha
Incubation time of 15 minutes on ice

controls

Positive control: Ionomycin was used to induce calcium mobilization in each cell line.

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