Transfection of Innate Immune Ligands

Transfections of 2′3′-cGAMP (Invivogen), htDNA (Sigma-Aldrich), and poly I:C (Sigma-Aldrich) were performed using Lipofectamine 2000 (Thermo Fisher Scientific) as previously described (Dobbs et al., 2015 (link)). After 6 h of treatment, RNA was isolated using Trizol (Sigma-Aldrich), and ISGs and IFN-β were measured via qRT-PCR.

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Warner J.D., Irizarry-Caro R.A., Bennion B.G., Ai T.L., Smith A.M., Miner C.A., Sakai T., Gonugunta V.K., Wu J., Platt D.J., Yan N, & Miner J.J. (2017). STING-associated vasculopathy develops independently of IRF3 in mice. The Journal of Experimental Medicine, 214(11), 3279-3292.

Publication 2017

2′3′-cGAMP htDNA poly I:C Lipofectamine 2000 Trizol

After treatment Cgamp Lipofectamine 2000 Poly i c Transfections Trizol

Corresponding Organization : The University of Texas Southwestern Medical Center

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Variable analysis

independent variables

2′3′-cGAMP (Invivogen)
HtDNA (Sigma-Aldrich)
Poly I:C (Sigma-Aldrich)

dependent variables

IFN-β

control variables

Lipofectamine 2000 (Thermo Fisher Scientific)
Treatment duration (6 h)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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