Polysaccharide Extraction and Fractionation

The polysaccharides were extracted as the method from Peng et al. with minor modification [15 (link)]. The crude cell wall material was suspended in 0.5% (w/v) ammonium oxalate and heated for 1 h in a boiling water bath, and the supernatants were combined as total pectin. The remaining pellet was suspended in 4 M KOH containing 1.0 mg mL^-1 sodium borohydride for 1 h at 25°C, and the combined supernatant was neutralized, dialyzed and lyophilized as hemicelluloses. The KOH non-extractable residue was further extracted with acetic-nitric acids for 1 h at 100°C and the remaining pellet was defined as crystalline cellulose. All samples were carried out in triplicate for wall fractionations.