Western Blotting Analysis of Protein Signaling

Cell extracts were separated by SDS-PAGE and proteins identified by western blotting, as previously described.^{15 (link)} Antibodies used for western blot detection were as follows: Axl (C-20), pEGFR, Akt 1/2/3 and pAkt 1/2/3 (Santa Cruz), EGFR (Cell Signalling Technology, Leiden, The Netherlands) and pAxl 779 (Bio-techne). Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-rabbit (Dako, Cambridge, UK), anti-goat and anti-mouse Igs (Promega, Southampton, UK).

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Vouri M., Croucher D.R., Kennedy S.P., An Q., Pilkington G.J, & Hafizi S. (2016). Axl-EGFR receptor tyrosine kinase hetero-interaction provides EGFR with access to pro-invasive signalling in cancer cells. Oncogenesis, 5(10), e266-.

Publication 2016

Akt 1/2/3 pAkt 1/2/3

Antibodies Cell extracts Egfr Goat Horseradish peroxidase Mouse Promega Proteins Rabbit Sds page Western blot

Corresponding Organization :

Other organizations : University of Portsmouth, Garvan Institute of Medical Research

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Variable analysis

independent variables

Cell extracts

dependent variables

Proteins identified by western blotting

control variables

Antibodies used for western blot detection
Secondary antibodies used

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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