Comprehensive Immunohistochemical Profiling of PDX Tumors

IHC assays were performed following established protocols [31 (link)]. After antigen retrieval (Tris-Cl, pH 9.0), paraffin-embedded sections of PDX tumors were incubated for 1 hour at room temperature with the following antibodies: antihuman CD45 (leukocyte common antigen, clones 2B11 + PD7/26); antihuman CD68, clone KP1; antihuman CD8 (clone C8/144B); antihuman CD4, clone 4B12; antihuman Ki-67, clone MIB-1 (Dako, Glostrup, Denmark); antihuman CD3, clone UCHT1 (STEMCELL Technologies); antihuman CD20, clone EP459Y; antihuman CD56, clone EPR2566 (Abcam, Cambridge, MA, USA); antihuman cytokeratin 19 (CK19), clone A53-B/A2.26, also known as Ks19.1 (Thermo Scientific, Waltham, MA, USA).

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Rosato R.R., Dávila-González D., Choi D.S., Qian W., Chen W., Kozielski A.J., Wong H., Dave B, & Chang J.C. (2018). Evaluation of anti-PD-1-based therapy against triple-negative breast cancer patient-derived xenograft tumors engrafted in humanized mouse models. Breast Cancer Research : BCR, 20, 108.

Publication 2018

antihuman CD45 antihuman CD68, clone KP1 antihuman CD8 (clone C8/144B); antihuman Ki-67, clone MIB-1

Antibodies Antigen Assays Clone Cytokeratin 19 Embedded paraffin Leukocyte common antigen Mib 1 Stemcell Tris Tumors

Corresponding Organization : Methodist Hospital

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Variable analysis

independent variables

Antibodies used for IHC assays: antihuman CD45, antihuman CD68, antihuman CD8, antihuman CD4, antihuman Ki-67, antihuman CD3, antihuman CD20, antihuman CD56, antihuman cytokeratin 19 (CK19)

dependent variables

Expression of CD45, CD68, CD8, CD4, Ki-67, CD3, CD20, CD56, and CK19 in PDX tumor sections

control variables

Tris-Cl, pH 9.0 used for antigen retrieval
Incubation time of 1 hour at room temperature for antibody staining

controls

No positive or negative controls were explicitly mentioned in the protocol.

Annotations

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