Cytotoxicity Assessment of Doxorubicin-Loaded Nanoparticles

2,4,6-Trimethoxybenzaldehyde was purchased from Acros Organics. 1,1,1-Tris(hydroxymethyl)ethane was purchased from Alfa Aesar. Methacryloyl chloride was purchased from Sigma Aldrich. The organic solvents dichloromethane, hexane, dimethylformamide, and methanol were analytical grade and purchased from Sigma Aldrich. Doxorubicin hydrochloride and fluorescein amine, isomer I were purchased from Sigma Aldrich. These chemicals were used without further purification. MDA-MB-231, A549, and NCI-H460 cell lines were purchased from ATCC (LGC Standards, Germany) and grown according to the culture method requirements of the manufacturer. Cells were incubated at 37 °C in a humidified atmosphere of 5% CO₂. Cytotoxicity experiments were performed with a plate reader (Multiscan FC, Thermo Scientific, USA) and Cell Counting Kit8 (CCK-8, Fluka) obtained from Sigma-Aldrich. The monomer was prepared according to previously reported literature.^{47 (link),56 (link)}