Visualizing poly(GA) protein fibrils in mouse brain
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Corresponding Organization :
Other organizations : Mayo Clinic in Florida, Jacksonville College, Johns Hopkins Medicine, Johns Hopkins University, USF Health Byrd Alzheimer's Institute, University of South Florida, Harvard University, Massachusetts General Hospital, German Center for Neurodegenerative Diseases, University of Colorado Boulder, Mayo Clinic
Variable analysis
- Pretreatment of thin sections with sodium citrate buffer, pH 6 at 90–95 °C for 10 min before immunolabeling
- Filamentous structure of poly(GA) proteins in mouse brain
- Rabbit polyclonal antibody to poly(GA) (1:150 in PBS) used as the primary antibody
- Goat anti-rabbit IgG conjugated with 18 nm colloidal gold particles (1:20, Jackson ImmunoResearch Laboratories) used as the secondary antibody
- Thin sections stained with uranyl acetate and lead citrate
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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