Reagents for Niemann-Pick Type C Studies

RG2833 and RGFP966 were purchased from Selleck Chemicals LLC (Houston, TX, USA). U18666A and GPN were obtained from Cayman Chemical Company (Ann Arbor, MI, USA). LysoTracker Red DND-99, LysoSensor Yellow/Blue DND-160 and Hoechst 33342 were from Molecular Probes/Invitrogen (Thermo Fisher Scientific, Darmstadt, Germany). GFP-certified FluoForte was from Enzo Life Sciences GmbH (Lörrach, Germany). Filipin III from streptomyces filipinensis, N,N-dimethylformamide and ApoA-I were purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). Bafilomycin A1 was obtained either from Calbiochem/Merck Millipore (Darmstadt, Germany) or from Tocris Bioscience (Bristol, UK). Fatty acid-free BSA was from PAA Laboratories (Pasching, Austria). [1,2-³H(N)]Cholesterol (1.813 TBq/mmol) was from PerkinElmer LAS (Rodgau, Germany). All other chemicals were from previously described sources18 (link)20 (link). The mouse NPC1-His6-EYFP expression plasmid (NPC1-YFP; Addgene plasmid 53523) was kindly provided by Dr. Matthew P. Scott via Addgene69 (link).