Polysomal profiling was performed according to previously described protocols (Bernabò et al, 2017 (link)). Briefly, cells were seeded in 10‐cm dishes, treated with CHX (10 µg/ml) for 4 min and then lysed in 300 µl of cold hypotonic lysis buffer [10 mM NaCl, 10 mM MgCl2•6H2O, 10 mM Tris–HCl, pH 7.5, 1% Triton X‐100, 0.2 U/μl Ribolock RNase inhibitor (Thermo Scientific), 0.0005 U/μl DNaseI (Thermo Scientific), CHX 10 μg/ml and 1 mM dithio‐threitol, 1% sodium deoxycholate]. The lysate was centrifuged at 4°C for 5 min at 1,620 × g to pellet cell debris. The cytoplasmic lysates loaded on a linear 10–40% [w/v] sucrose gradient and centrifuged in a SW41Ti rotor (Beckman) for 1 h 30 min at 187,813 × g at 4°C in a Beckman Optima XPN‐100 Ultracentrifuge. Fractions of 1 ml of volume were then collected monitoring the absorbance at 254 nm with the UA‐6 UV/VIS detector (Teledyne Isco).