CHO (Gu et al., 1999 (link); Xi et al., 2003 (link)), 293FT, and GD25 cells were cultured in DMEM complete (Cellgro) supplemented with 10% fetal bovine serum (FBS; Corning), 2 mM l -glutamine (Corning), 100 U/ml penicillin plus 100 μg/ml streptomycin (Corning), 1 mM sodium pyruvate (Gibco), 0.1 mM nonessential amino acids (Gibco), and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (Gibco). Cells were serum starved in DMEM containing each of the foregoing components except the FBS for at least 4 h before experiments.
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Cell Culture Conditions for CHO, 293FT, and GD25 Cells
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Other organizations : University of Illinois at Chicago, The Ohio State University, University of Wisconsin–Madison
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Variable analysis
independent variables
- Cell types: CHO (Gu et al., 1999 (link); Xi et al., 2003 (link)), 293FT, and GD25 cells
- Not explicitly mentioned
- Culture media: DMEM complete (Cellgro) supplemented with 10% fetal bovine serum (FBS; Corning), 2 mM L-glutamine (Corning), 100 U/ml penicillin plus 100 μg/ml streptomycin (Corning), 1 mM sodium pyruvate (Gibco), 0.1 mM nonessential amino acids (Gibco), and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (Gibco)
- Serum starvation: Cells were serum starved in DMEM containing each of the foregoing components except the FBS for at least 4 h before experiments
- Positive control: Not specified
- Negative control: Not specified
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