Liquid
cells were prepared
as previously described in the literature.71 (link) Briefly, solutions of ethanol and trans-anethole
were drop cast onto nonglow discharged SiNx chips in amounts less than 0.8 μL. Top chips were deposited
such that the windows were aligned orthogonally, and the holder was
sealed with the top clamp assembly. Lines were left unfilled with
solvent so as to avoid premature dilution of the sample with diluent.
The holder was then pumped down using the external pumping station,
and the cell windows were visually inspected using the attached optical
microscope. Once the cell has reached 8.6 × 10–6 mbar, the fluidic ports were unsealed and the flow line was attached,
so as to ensure cell integrity during dilution. This flow line was
attached to a syringe and syringe pump, which were used to flow in
water to the cell at rates from 1 to 5 μL/min. A JEM-ARM300F
transmission electron microscope operating at a voltage of 300 keV
and current of 15 μA (FEG source) was used for liquid cell experiments.
Images were acquired via Gatan 2k × 2k OneView IS CMOS camera via Gatan Digital Micrograph imaging
software with exposures of 1 s.
cells were prepared
as previously described in the literature.71 (link) Briefly, solutions of ethanol and trans-anethole
were drop cast onto nonglow discharged SiNx chips in amounts less than 0.8 μL. Top chips were deposited
such that the windows were aligned orthogonally, and the holder was
sealed with the top clamp assembly. Lines were left unfilled with
solvent so as to avoid premature dilution of the sample with diluent.
The holder was then pumped down using the external pumping station,
and the cell windows were visually inspected using the attached optical
microscope. Once the cell has reached 8.6 × 10–6 mbar, the fluidic ports were unsealed and the flow line was attached,
so as to ensure cell integrity during dilution. This flow line was
attached to a syringe and syringe pump, which were used to flow in
water to the cell at rates from 1 to 5 μL/min. A JEM-ARM300F
transmission electron microscope operating at a voltage of 300 keV
and current of 15 μA (FEG source) was used for liquid cell experiments.
Images were acquired via Gatan 2k × 2k OneView IS CMOS camera via Gatan Digital Micrograph imaging
software with exposures of 1 s.
Free full text: Click here
