Cell Culture Protocols for Neuroscience

HEK293 (ATCC CRL-1573) and DI TNC1 (ATCC CRL-2005) and BV2 cell lines were cultured in D10 (DMEM-10% FBS with 1X Pen/Strep). Schwann cells harvested from adult rat sciatic nerves [24 (link)] were seeded on poly-L-lysine (PLL) coated petri dishes in D10-3M media (D10, 2 μM Forskolin, 10 nM Heregulin, and 20 μg/mL of pituitary extract). Rat astrocytes isolated from E18-19 rat cortices [25 (link)] were cultured in astrocyte medium (A1261301, Gibco, Carlsbad, CA) and grown in 75 cm² vented culture flasks. All cell cultures were maintained inside a humidified incubator at 37°C and 5% CO₂.