Prostate Cell Line Authentication and Culture Protocols

RWPE-1, RWPE-2, PC-3 and LNCaP were obtained from American Type Culture Collection (ATCC) in 2013 and re-authenticated in 2016 (Supplemental Document for Review purposes). RWPE-1 and RWPE-2 cells were cultured in KSFM with bovine pituitary extract and epidermal growth factor (ThermoScientific, Waltham, MA), and used passage <21. Primary human benign epithelial cells (PrE) and organoids were grown in PrEGM media (Lonza, ThermoScientific). The PrE cells were established from fresh radical prostatectomy tissues, as previously described^{19 (link), 44 (link)}, via UIC Institutional Review Board approved protocols (#12–0331 and 15–1294). PC-3 were cultured in DMEM with 10% fetal bovine serum (FBS) and LNCaP in RPMI with 10% FBS.

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Dambal S., Baumann B., McCray T., Williams L., Richards Z., Deaton R., Prins G.S, & Nonn L. (2017). The miR-183 family cluster alters zinc homeostasis in benign prostate cells, organoids and prostate cancer xenografts. Scientific Reports, 7, 7704.

Publication 2017

RWPE-1 RWPE-2 LNCaP PrEGM media

Bovine Cells Epidermal growth factor Epithelial cells Fetal bovine serum Human Institutional review board Organoids Prostatectomy Tissues

Corresponding Organization :

Other organizations : University of Illinois at Chicago

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Variable analysis

independent variables

Cell lines (RWPE-1, RWPE-2, PC-3, LNCaP, PrE cells, organoids)

dependent variables

Not explicitly mentioned

control variables

Cell passage number (<21 for RWPE-1 and RWPE-2 cells)
Culture media (KSFM with bovine pituitary extract and epidermal growth factor for RWPE-1 and RWPE-2 cells, DMEM with 10% FBS for PC-3 cells, RPMI with 10% FBS for LNCaP cells, PrEGM media for PrE cells and organoids)

controls

Positive control: Primary human benign epithelial (PrE) cells and organoids
Negative control: Not explicitly mentioned

Annotations

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