Cell Differentiation and Maturation Analysis

In this study, the maturation of cells to the different types of differentiation was verified by the observation of the cell morphology by the staining cyto‐centrifuged cells using a Shandon 3 Cytospin (Thermo Electron) with the Hemacolor kit from Merck and light microscope examination. Additionally, characteristic of cell maturation into macrophages was assessed by the analysis of different related markers like CD11b using the flow cytometer analyzer (Coulter‐Beckman). For that, cells (during 3 days at 2 × 10⁵ cells/ml) were immunostained with an appropriate antibody (Abs) against CD11b marker and the percentages of positive cells and antigen relative density per cell was measured (Merhi et al., 2008 (link)). The percentage was obtained by subtracting the peak channel number of the negative control from the peak channel number of the corresponding experimental sample.