Chemotherapy Cytotoxicity Evaluation via Acid Phosphatase

To determine the cytotoxicity of chemotherapy drugs, cell growth/viability was measured using an acid phosphatase assay; 1.5–3 × 10³ cells were seeded in flat-bottomed 96-well plates and incubated overnight prior to addition of drug. Chemotherapeutics were obtained from St Vincent’s University Hospital, Dublin, Ireland. Lapatinib was purchased from Sequoia. Temozolomide was obtained from the National Cancer Institute. Other inhibitors and modulators were obtained from Sigma. Drug-free controls were included in each assay. Plates were incubated for a further 5 (HCC1954, Malme-3M and HT144) or 7 days (H1299 and H460) at 37°C in a humidified atmosphere with 5% CO₂ and cell viability was determined using an acid phosphatase assay (97 (link)). Growth of drug-treated cells was calculated relative to control untreated cells in biological triplicate.