Inhibition of Human p97 ATPase Activity
Corresponding Organization : University of New Mexico
Other organizations : University of Kansas Medical Center, UCLA Medical Center, The Lundquist Institute, Harbor–UCLA Medical Center
Variable analysis
- Compound concentration (in 8-dose titration)
- Inhibition of human p97 ATPase activity
- Assay buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 1 mM EDTA, 0.5 mM TCEP)
- 0.01% Triton X-100
- 200 μM ATP
- Human p97 (25 nM monomer)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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