Single-cell Analysis of Transplanted Lungs

Lungs harvested 24 hours following orthotopic lung transplantation were processed to a single-cell suspension and were stained according to a previously described method (69 (link)). Antibodies used for lung staining can be found in Supplemental Table 1. Samples were run on a BD FACSymphony A5-Laser Analyzer at the Northwestern University Robert H. Lurie Comprehensive Cancer Center Flow Cytometry Core Facility and then subsequently analyzed with FlowJo v10.6.2 (FlowJo).

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Querrey M., Chiu S., Lecuona E., Wu Q., Sun H., Anderson M., Kelly M., Ravi S., Misharin A.V., Kreisel D., Bharat A, & Budinger G.R. (2022). CD11b suppresses TLR activation of nonclassical monocytes to reduce primary graft dysfunction after lung transplantation. The Journal of Clinical Investigation, 132(14), e157262.

Publication 2022

BD FACSymphony A5-Laser Analyzer

Antibodies Cancer Flow cytometry Lung Lung transplantation

Corresponding Organization : Pulmonary and Critical Care Associates

Other organizations : Washington University in St. Louis

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Variable analysis

independent variables

Time following orthotopic lung transplantation

dependent variables

Cell types present in the lung tissue sample

control variables

Lung tissue processing method
Staining protocol
Flow cytometry instrumentation and analysis software

controls

Previously described staining method (Supplemental Table 1)

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