Paraffin-embedded tumor sections (n = 8/group; Three micrometer thick) were processed with the Tumor TACS In Situ Apoptosis Detection kit (Trevigen)9 (link) or with Click-iT TUNEL Colorimetric ICH Detection kit (Invitrogen) according to manufacturer’s protocols. Apoptotic nuclei labeled by diaminobenzidine were counted on 10 squares/sample (n = 8/group). Magnification 20 × .
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