DLS Analysis of LE-MixNano Particles

The average hydrodynamic diameter (D_h) and polydispersity index (PI) of LE-MixNano were determined using dynamic light scattering (DLS) analysis (Beckman Coulter^® N4 Plus, Beckman Coulter s.r.l, Milan, Italy). Five minutes before the DLS measurements, each sample was adequately diluted with ultrapure water, previously filtered through a 0.45 μm pore size filter (Phenex^® RC Syringe filter, Phenomenex, Torrance, CA, USA), choosing the final concentration to reach a measurement intensity ranging from 5 × 10⁴ to 1 × 10⁶ counts per second (cps). D_h and PI for each LE-MixNano preparation were measured at 20 °C with 6 runs of three different samples at an angle of 90° [35 (link)] and a run time of 200 s.