Investigating AANG Effects on mTECs

The mouse tubular epithelial cells (mTEC) were cultured in DMEM/F12 (Gibco, CA), supplemented with 10% FBS (Gibco, CA) and 1% antibiotic/antimycotic solution (Life Technologies, USA). The mouse mesangial cells (mMES) (MES13 ATCC, Manassas, VA, USA) was maintained in a 3:1 mixture of DMEM and Ham's F-12 medium containing 5% FBS, and 1% antibiotic/antimycotic solution (Life Technologies, Grand Island, NY, USA)10 (link), 12 (link), 15 (link). mTECs were seeded and treated with combination of AANG (20 µM AA+200 µM NG) in a 6-well plate for 6-hours, then stimulated with advanced glycation end-products (AGE) for 24-hours. Subsequently, stimulated mTECs were employed for western blot analysis.

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Chung J.Y., Tang P.M., Chan M.K., Wang L., Huang X.R., To K.F., Ma R.C, & Lan H.Y. (2022). AANG Prevents Smad3-dependent Diabetic Nephropathy by Restoring Pancreatic β-Cell Development in db/db Mice. International Journal of Biological Sciences, 18(14), 5489-5502.

Publication 2022

DMEM/F12 FBS antibiotic/antimycotic solution DMEM

Advanced glycation end products Antibiotic Epithelial cells Mesangial cells Mouse Western blot analysis

Corresponding Organization :

Other organizations : Chinese University of Hong Kong, Southwest Medical University, Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital

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Variable analysis

independent variables

Combination of AANG (20 µM AA+200 µM NG)

dependent variables

Protein expression/levels (measured by western blot analysis)

control variables

Culture medium (DMEM/F12, 3:1 mixture of DMEM and Ham's F-12 medium)
Serum concentration (10% FBS, 5% FBS)
Antibiotic/antimycotic solution (1%)

controls

Negative control: Not explicitly mentioned
Positive control: Not explicitly mentioned

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