Ion PGM Sequencing Library Preparation

The sequencing procedure was described in detail in the previous publication [6 (link)]. The DNA library was constructed using the Ion Plus Fragment Library Kit (Life Technologies, Carlsbad, CA, USA) and purified using the Agencourt AMPure XP Reagent (Beckman Coulter, Brea, CA, USA) and then quantified by the Ion Universal Library Quantitation Kit and a real-time PCR instrument—Quant Studio 5 (Life Technologies, Carlsbad, CA, USA). The library was coated onto beads and sequenced using an Ion PGM System using the Ion PGM™ Hi-Q™ View Sequencing Kit on an Ion 316™ Chip Kit v2 BC (Life Technologies, Carlsbad, CA, USA).

Free full text: Click here

Staninska-Pięta J., Czarny J., Wolko Ł., Cyplik P., Drożdżyńska A., Przybylak M., Ratajczak K, & Piotrowska-Cyplik A. (2023). Temperature, Salinity and Garlic Additive Shape the Microbial Community during Traditional Beetroot Fermentation Process. Foods, 12(16), 3079.

Publication 2023

Ion Plus Fragment Library Kit Agencourt AMPure XP Reagent Ion Universal Library Quantitation Kit —Quant Studio 5 Ion PGM™ Hi-Q™ View Sequencing Kit

Library Real time pcr

Corresponding Organization : University of Life Sciences in Poznań

Top 5 similar protocols

Variable analysis

independent variables

DNA library construction method (Ion Plus Fragment Library Kit)
Library purification method (Agencourt AMPure XP Reagent)
Library quantification method (Ion Universal Library Quantitation Kit and Quant Studio 5 real-time PCR instrument)
Sequencing platform (Ion PGM System)
Sequencing kit (Ion PGM™ Hi-Q™ View Sequencing Kit)
Sequencing chip (Ion 316™ Chip Kit v2 BC)

dependent variables

Sequencing results

control variables

Not explicitly mentioned

controls

Not specified
Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!