Genomic DNA was extracted from peripheral blood cells (PBCs) and tissue samples using the QIAamp Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions, and quantified on a Bio Spectrometer Plus (Eppendorf, Hamburg, Germany). The entire coding region of the PIK3CA gene was sequenced and analyzed according to the methods indicated in our previous report [11 (link)].
Free full text: Click here