PIK3CA Gene Sequencing from PBCs
Corresponding Organization :
Other organizations : University of Bari Aldo Moro, Gastroenterology Hospital "Saverio de Bellis", Bambino Gesù Children's Hospital, Azienda Socio Sanitaria Territoriale Lariana, Presidio Ospedaliero, University of Naples Federico II, Istituto Giannina Gaslini, University of Catania, King's College London, Arqule (United States)
Variable analysis
- Extraction of genomic DNA from peripheral blood cells (PBCs) and tissue samples
- Sequencing and analysis of the entire coding region of the PIK3CA gene
- Use of the QIAamp Mini Kit (Qiagen, Hilden, Germany) for DNA extraction according to the manufacturer's instructions
- Quantification of DNA on a Bio Spectrometer Plus (Eppendorf, Hamburg, Germany)
- No positive or negative controls were explicitly mentioned in the input protocol.
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