Phenotypic Characterization of Mesenchymal Stem Cells

cMSCs and pbMSCs were defined according to the 3 criteria of the International Society for Cellular Therapy [13 (link)]: (1) adhesion to plastic, (2) expression of a specific combination of surface markers, and (3) differentiation potential (trilineage differentiation into adipocytes, osteoblasts, chondrocytes, and blood vascular cells) [10 (link)]. To define the phenotype of cMSCs and pbMSCs, we analyzed cultured cells at passage 4 for rat MSC markers by flow cytometry using the following fluorescence antibodies: SH2, SH3, CD90, CD147, CD34, CD45, and CD133. Mouse IgG1, IgG2a, and IgG2b (Becton Dickinson) were used as isotype controls, and marker expression was evaluated using FACS.

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Zhao L., Wang J., Wang P., Deng Z., Cui J., Huang W, & Zhang S. (2022). Oct4 cooperates with c-Myc to improve mesenchymal-to-endothelial transition and myocardial repair of cardiac-resident mesenchymal stem cells. Stem Cell Research & Therapy, 13, 445.

Publication 2022

Mouse IgG1 IgG2a IgG2b

Adipocytes Blood cells Cd147 Cd90 Cellular therapy Chondrocytes Cultured cells Flow cytometry Fluorescence antibodies Igg1 Igg2a Igg2b Isotype Mouse Osteoblasts Phenotype

Corresponding Organization :

Other organizations : Nanfang Hospital, Jinan University

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Variable analysis

independent variables

Cultured cell type (cMSCs or pbMSCs)

dependent variables

Expression of rat MSC markers (SH2, SH3, CD90, CD147, CD34, CD45, CD133)

control variables

Passage 4 of cultured cells
Use of mouse IgG1, IgG2a, and IgG2b (Becton Dickinson) as isotype controls

controls

Positive control: Not explicitly mentioned
Negative control: Mouse IgG1, IgG2a, and IgG2b (Becton Dickinson) used as isotype controls

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