Autophagy Modulation in Cell Lines

Cells were seeded in 6-well plates 24 h prior to any treatment with the following compounds: bafilomycin A1 (BAF.A1, LC Laboratories, Woburn, MA, USA, R-5000, B-1080, 100 nM) [18 (link)]; rapamycin (RAPA, Fisher, Pittsburgh, PA, USA, BP2963.1, 1 µΜ) [19 (link)], Hegf (E9644, 100 ng/Ml), pepstatin A (P5318, 50–100 µM), DMSO as a vehicle (dilution factor 1:1000), all from Sigma-Aldrich; and leupeptin (Enzo, ALX-260–009, Farmingdale, NY, USA, 50–100 µM).

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Alegre-Cortés E., Giménez-Bejarano A., Uribe-Carretero E., Paredes-Barquero M., Marques A.R., Lopes-da-Silva M., Vieira O.V., Canales-Cortés S., Camello P.J., Martínez-Chacón G., Aiastui A., Fernández-Torrón R., López de Munain A., Gomez-Suaga P., Niso-Santano M., González-Polo R.A., Fuentes J.M, & Yakhine-Diop S.M. (2022). Delay of EGF-Stimulated EGFR Degradation in Myotonic Dystrophy Type 1 (DM1). Cells, 11(19), 3018.

Publication 2022

bafilomycin A1 (BAF.A1, Hegf pepstatin A leupeptin

Bafilomycin a1 Cells Dilution Dmso Factor 1 Leupeptin Pepstatin Rapamycin

Corresponding Organization : Biomedical Research Networking Center on Neurodegenerative Diseases

Other organizations : Universidade Nova de Lisboa, Biogipuzkoa Health Research Institute, Osakidetza, University of the Basque Country

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Variable analysis

independent variables

Bafilomycin A1 (BAF.A1, 100 nM)
Rapamycin (RAPA, 1 µΜ)
Hegf (100 ng/Ml)
Pepstatin A (50–100 µM)
DMSO (1:1000 dilution)
Leupeptin (50–100 µM)

dependent variables

Not explicitly mentioned

control variables

Cells were seeded in 6-well plates 24 h prior to any treatment

controls

DMSO as a vehicle (dilution factor 1:1000)

Annotations

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