Tissue (hearts, livers, spleens, duodenums, brains, right lungs and kidneys) were fixed in 4% paraformaldehyde and were processed for paraffin embedding. The 4-μm sections were stained with hematoxylin and eosin for histopathological examinations. For immunohistochemistry, SARS-CoV-2 N protein was detected using monoclonal antibody (4D11)34 (link), CD3 was detected using polyclonal rabbit anti-human CD3 antibodies (DAKO), and the neuron-specific beta-III tubulin was detected using monoclonal antibody clone TuJ1 (R&D Systems). Images were captured using a Leica DFC 5400 digital camera and were processed using Leica Application Suite v4.13.
Protocol detail
Histopathological Analysis of SARS-CoV-2 in Tissues
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Anti cd3
Antibodies
Beta tubulin
Brains
Camera
Clone
Duodenums
Eosin
Examinations
Hearts
Human
Immunohistochemistry
Kidneys
Livers
Lungs
Monoclonal antibody
Neuron
Paraformaldehyde
Rabbit
Sars cov 2 n protein
Tissue
Corresponding Organization :
Other organizations : University of Hong Kong
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Variable analysis
independent variables
- Tissue type (hearts, livers, spleens, duodenums, brains, right lungs and kidneys)
- Histopathological examination
- SARS-CoV-2 N protein detection
- CD3 detection
- Neuron-specific beta-III tubulin detection
- Paraformaldehyde fixation
- Paraffin embedding
- 4-μm section thickness
- Hematoxylin and eosin staining
- Monoclonal antibody (4D11) for SARS-CoV-2 N protein detection
- Polyclonal rabbit anti-human CD3 antibodies (DAKO) for CD3 detection
- Monoclonal antibody clone TuJ1 (R&D Systems) for neuron-specific beta-III tubulin detection
- Leica DFC 5400 digital camera for image capture
- Leica Application Suite v4.13 for image processing
- Not explicitly mentioned
- Not explicitly mentioned
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