Immunoblotting of EMT and m6A Regulators

Immunoblotting was carried out as previously described [44 (link)]. Antibodies against E-cadherin (3195, 1:1000), Vimentin (5741, 1:1000), p-STAT3 (9145, 1:1000), ZEB1 (70512, 1:1000) and β-actin (4970, 1:1000) were purchased from Cell Signaling Technology. Antibodies against FTO (ab126605, 1:1000), YTHDF1 (ab252563, 1:1000), YTHDF2 (ab246514, 1:1000), YTHDF3 (ab220161, 1:1000), TSG101 (ab125011, 1:1000), and CD81 (ab134045, 1:1000) were purchased from Abcam. Antibodies against IGF2BP1 (22803-1-AP, 1:1000), IGF2BP2 (11601-1-AP, 1:1000), IGF2BP3 (14642-1-AP, 1:1000) and GAPDH (60004-1-Ig, 1:2000) were purchased from Proteintech (China). Full and uncropped blots were uploaded as Supplemental Material.