The cells were treated with perifosine as described for the MTT assay. Quantification of cellular ATP was performed in cells cultured in 24-well plates using the ATP assay kit (Cayman Chemicals, Ann Arbor, Michigan, USA) as described earlier (53 (link)). The ATP concentration was subtracted from the calibration curve. The 3D models were disintegrated by trypsin and subjected to ATP assay using the same procedure.
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