Culturing Human Gastric Cancer Organoids

The human organoid culture was performed as our previously reported [28 (link)]. Sterile gastric cancer tissues were cut into small pieces and digested with collagenase A. Then, the Matrigel (R&D Systems, USA) supplemented with growth factors was used to resuspend cells. After that, Organoid Growth Medium (human) (StemCell Technologies, Canada) was used to culture the mixtures that were seeded in a 24-well plate. Photographs of human GC organoids were taken daily by microscope.

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Li Z., Fan H., Cao J., Sun G., Sen W.a.n.g., Lv J., Xuan Z., Xia Y., Wang L., Zhang D., Xu H, & Xu Z. (2021). Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process. Cell Death & Disease, 12(11), 968.

Publication 2021

Matrigel Organoid Growth Medium (human)

Cells Collagenase Gastric cancer Growth factors Growth medium Human Matrigel Microscope Organoids Stemcell Sterile Tissues

Corresponding Organization : Nanjing Medical University

Other organizations : Jiangsu Province Hospital

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Variable analysis

independent variables

Cutting gastric cancer tissues into small pieces
Digesting with collagenase A

dependent variables

Growth of human GC organoids

control variables

Use of Matrigel (R&D Systems, USA) supplemented with growth factors to resuspend cells
Use of Organoid Growth Medium (human) (StemCell Technologies, Canada) to culture the mixtures

controls

No positive or negative controls were explicitly mentioned in the protocol.

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