Immunohistochemical Analysis of Dopaminergic Neurons

For histological analysis, the mice were intracardially perfused with 0.9% NaCl and fixed with 4% paraformaldehyde in 0.1 M PBS (pH 7.4). Cryoprotected brains were serially sectioned at 40 μm in the coronal plane using a freezing microtome (MICROM; Walldorf, Germany). The sections were immunostained for STR and substantia nigra (SN) using anti-tyrosine hydroxylase (TH; a dopaminergic neuronal marker) as previously described [24 (link)]. Images were acquired using a Nikon ECLIPSE TE 200-U microscope (Tokyo, Japan).

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Thirumalai D., Lee S., Kwon M., Paik H.J., Lee J, & Chang S.C. (2021). Disposable Voltammetric Sensor Modified with Block Copolymer-Dispersed Graphene for Simultaneous Determination of Dopamine and Ascorbic Acid in Ex Vivo Mouse Brain Tissue. Biosensors, 11(10), 368.

Publication 2021

ECLIPSE TE 200-U microscope

0 9 nacl Brains Dopaminergic neuronal Mice Microscope Microtome Paraformaldehyde Substantia nigra Tyrosine hydroxylase

Corresponding Organization : Pusan National University

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Variable analysis

independent variables

Intracardial perfusion with 0.9% NaCl
Fixation with 4% paraformaldehyde in 0.1 M PBS (pH 7.4)

dependent variables

Immunostaining for STR and substantia nigra (SN) using anti-tyrosine hydroxylase (TH; a dopaminergic neuronal marker)

control variables

Cryoprotected brains serially sectioned at 40 μm in the coronal plane using a freezing microtome
Imaging using a Nikon ECLIPSE TE 200-U microscope

controls

Positive control: None specified
Negative control: None specified

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