The copper chelation test was performed as described previously [51 (link)]. Briefly, 96-well microplates with a reaction mixture containing different concentrations of samples (0.1–2 mg/mL), pyrocatechol violet (4 mM) and copper II sulfate pentahydrate (50 mg/mL) were homogenized with the aid of a micropipette, and the absorbance of the solution was measured at 632 nm using a microplate reader (SpectraMax® M2/M2e, Molecular Devices, São José, CA, USA).
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