Comprehensive Antibody Toolkit for TG2 Analysis

Chemical reagents were mainly purchased from WAKO chemicals (Osaka, Japan) and Nacalai Tesque (Kyoto, Japan). Primary and fluorescein-conjugated secondary antibodies were listed in Suppl. Table S1. Polyclonal anti-TG2 antibody was produced in our laboratory [29 (link)]. HRP-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Cystamine was obtained from Sigma-Aldrich (St. Louis, USA). Z-DON and Boc-DON were obtained from Zedira (Darmstadt, Germany). PD146176 and 15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid, 15(S)-HETE, were purchased from Cayman Chemical (Ann Arbor, MI, USA).

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Shinoda Y., Tatsukawa H., Yonaga A., Wakita R., Takeuchi T., Tsuji T., Tanaka M., Suganami T, & Hitomi K. (2023). Tissue transglutaminase exacerbates renal fibrosis via alternative activation of monocyte-derived macrophages. Cell Death & Disease, 14(2), 136.

Publication 2023

HRP-conjugated secondary antibodies Cystamine PD146176

Anti antibody Antibodies Cayman Cystamine Eicosatetraenoic acid Fluorescein Hete

Corresponding Organization :

Other organizations : Nagoya University

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Variable analysis

independent variables

Chemical reagents purchased from WAKO chemicals (Osaka, Japan) and Nacalai Tesque (Kyoto, Japan)
Primary and fluorescein-conjugated secondary antibodies
Polyclonal anti-TG2 antibody produced in the authors' laboratory
HRP-conjugated secondary antibodies obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA)
Cystamine obtained from Sigma-Aldrich (St. Louis, USA)
Z-DON and Boc-DON obtained from Zedira (Darmstadt, Germany)
PD146176 and 15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid, 15(S)-HETE, purchased from Cayman Chemical (Ann Arbor, MI, USA)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Polyclonal anti-TG2 antibody produced in the authors' laboratory

negative controls

Not explicitly mentioned

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