Neutrophil Depletion for BSA-NP Study

To deplete neutrophils, mice were treated with a 400 μg intraperitoneal injection of anti-mouse Ly6G antibody (108454, Biolegend) or IgG2b isotype control (400675, Biolegend) 24 h prior to injection of BSA-NPs. Subsequent 200 μg antibody injections were administered accompanied by BSA-NPs. Animals were euthanized after 16 h to collect peritoneal lavage fluid, ectopic lesions, and eutopic endometrium. Neutrophil depletion was confirmed by flow cytometry as described below.

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Zhu S., Zhang J., Xue N., Zhu X., Li F., Dai Q., Qing X., Chen D., Liu X., Wei Z, & Cao Y. (2023). Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. Journal of Nanobiotechnology, 21, 81.

Publication 2023

anti-mouse Ly6G antibody IgG2b isotype control

Animals Antibody injections Endometrium Flow cytometry Igg2b Isotype Mouse Neutrophil Peritoneal fluid Peritoneal lavage

Corresponding Organization : First Affiliated Hospital of Anhui Medical University

Other organizations : Hefei National Center for Physical Sciences at Nanoscale, University of Science and Technology of China

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Variable analysis

independent variables

Anti-mouse Ly6G antibody (108454, Biolegend)
IgG2b isotype control (400675, Biolegend)

dependent variables

Neutrophil depletion
Peritoneal lavage fluid
Ectopic lesions
Eutopic endometrium

control variables

Time of antibody injection (24 h prior to BSA-NPs injection)
Subsequent 200 μg antibody injections accompanied by BSA-NPs
Time of euthanasia (16 h after injection)

controls

Positive control: Anti-mouse Ly6G antibody
Negative control: IgG2b isotype control

Annotations

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