Extracting Genomic DNA from Fungal Isolates

Isolates were cultured on PDA and harvested and ground using liquid N₂. Genomic DNA was extracted using a cetyltrimethyl ammonium bromide (CTAB) protocol [47 (link),48 (link)]. DNA samples were assessed by running on a 1% agarose gel for the presence of DNA shearing and RNA, while DNA integrity was evaluated using TapeStation and Genomic DNA ScreenTapes (Agilent, Santa Clara, CA, USA) and impurities were evaluated using DropSense 16 (Trinean, Pleasantan, CA, USA). gDNA was quantified using a Qubit^® 2.0 Fluorometer (Invitrogen by Life Technologies, Carlsbad, CA, USA) before submission for in-house sequencing (Molecular Technologies Laboratory, Ottawa Research & Development Centre, Agriculture and Agri-Food Canada) where DNA libraries were prepared and loaded onto a FLO-MIN106 flow cell and run with a MinION (Oxford Nanopore Technologies, Oxford, UK) for 48 h.