HSC-T6 cells were extracted twice with hexane/isopropanol (3/2, v/v) for 10 min under constant shaking. The organic phases were combined, evaporated, and lipids were dissolved in chloroform/methanol (2/1, v/v). TGs and CEs were separated on a Betasil® Diol Column (100 × 4.6 mm, 5 μm; Thermo Fisher Scientific, Waltham, MA) using a ternary gradient solvent system and detected by HPLC-ELSD as described (25 (link)). The HPLC-ELSD consisted of a precooled sample manager (at 4°C), pump, injector, and column oven (at 40°C), all of the Agilent 1100 Series (Santa Clara, CA) and were coupled to a Sedex 85 evaporative light scattering detector (Sedere, Alfortville, France). Data were analyzed using the Chemstation software (B 04.01; Agilent, Santa Clara, CA). REs were separated on a YMC-Pro C18 column (150×4.6mm, S-5μm, 12nm; YMC Europe GmbH, Dinslaken, Germany) using a isocratic solvent system (98% methanol, 2% water, 1.2 ml/min) and detected at excitation 325 nm/emission 450 nm. The HPLC consisted of a Waters e2695 Separation Module, including a column oven (at 35°C), and a Waters 2475 Fluorescence Detector (Waters Corporation, Milford, MA). Data were analyzed using Empower 3 Chromatography Data Software (Waters Corporation). Neutral lipid standards were prepared as 1 mg/ml stock solutions in chloroform/methanol 2:1 (v/v). Calibration curves were measured from 2.7 μg/ml to 350 μg/ml.