Individual Vβ clones were detected in PBMC using TCR-Vβ repertoire kit from Beckman Coulter as per manufacturer’s instructions. Cells were stained simultaneously with for CD3 and IFN-γ, and a set of three antibodies directed against TCR-Vβ repertoires and analyzed by flow cytometry. Cells were gated on CD3+cells, individual TCR families were then analyzed for IFN-γ positive CD3+ T cells.
Staining for HER2 BATs in the peripheral blood. The percentage of anti-CD3 x anti-HER2 positive cells was quantitated by using goat PE conjugated anti-mouse IgG2a antibodies to detect OKT3 in the BiAb as reported previously [21 (link)].
Staining for HER2 BATs in the peripheral blood. The percentage of anti-CD3 x anti-HER2 positive cells was quantitated by using goat PE conjugated anti-mouse IgG2a antibodies to detect OKT3 in the BiAb as reported previously [21 (link)].
