Serum Androgen and Corticosterone Levels

The level of androgens, in serum, was referred to as T+DHT considering that the anti-testosterone serum №250 showed 100% cross-reactivity with DHT (for references, please see [20 (link),22 (link)]). Serum androgen levels were measured via radioimmunoassay. All samples were measured in duplicate in one assay (sensitivity: 6 pg per tube; intra-assay coefficient of variation 5–8%). Serum corticosterone (CORT) levels in all samples were measured in duplicate in one assay using the corticosterone EIA Kit (Cayman Chemical, Michigan, MI, USA) with 30 pg/mL as the lowest standard significantly different from blank.

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Starovlah I.M., Radovic Pletikosic S.M., Tomanic T.M., Medar M.L., Kostic T.S, & Andric S.A. (2022). Spermatozoal Mitochondrial Dynamics Markers and Other Functionality-Related Signaling Molecules Exert Circadian-like Response to Repeated Stress of Whole Organism. Cells, 11(6), 993.

Publication 2022

corticosterone EIA Kit

Androgen Assay Cayman Corticosterone Cross reactivity Radioimmunoassay Sensitivity Serum Testosterone

Corresponding Organization : University of Novi Sad

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum androgen levels (T+DHT)
Serum corticosterone (CORT) levels

control variables

All samples were measured in duplicate in one assay
Sensitivity: 6 pg per tube
Intra-assay coefficient of variation 5–8%
Lowest standard for CORT significantly different from blank (30 pg/mL)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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