Examining Phosphorylation Signaling Pathways

The following antibodies were purchased from Cell Signaling Technologies: pNDRG1, pPDK1, pAKT308, pAKT473, pGSK3β (Ser 9), pPRAS40 (Thr 246), pFOXO3a (Thr 32), pERK1/2, AKT, FOXO3a, pP70S6K (Thr 389), pS6K (Ser 235/236), PRAS40, GSK3b, ERK1/2, SGK3, cyclin D1, PTEN, P70S6K, and S6. Antibodies against β-actin, SGK1, PKC, and tubulin were purchased from Santa Cruz Biotechnology. 4-Hydroxytamoxifen and antibodies against S100 were purchased from Sigma. Synthesis of the PDK1 inhibitor GSK2334470 was performed as previously described (5 (link)).

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Scortegagna M., Lau E., Zhang T., Feng Y., Sereduk C., Yin H., De S.K., Meeth K., Platt J.T., Langdon C.G., Halaban R., Pellecchia M., Davies M.A., Brown K., Stern D.F., Bosenberg M, & Ronai Z.A. (2015). PDK1 and SGK3 contribute to the growth of BRAF mutant melanomas and are potential therapeutic targets. Cancer research, 75(7), 1399-1412.

Publication 2015

pNDRG1 pPDK1 pAKT308 pAKT473 FOXO3a PRAS40 GSK3b ERK1/2 P70S6K Antibodies against β-actin 4-Hydroxytamoxifen

4 hydroxytamoxifen Actin Antibodies Cyclin d1 Erk1 Gsk2334470 P70s6k Pdk1 Pten S100 Synthesis Tubulin

Corresponding Organization : Sanford Burnham Prebys Medical Discovery Institute

Other organizations : Translational Genomics Research Institute, Yale University, The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

4-Hydroxytamoxifen

dependent variables

PNDRG1
PPDK1
PAKT308
PAKT473
PGSK3β (Ser 9)
PPRAS40 (Thr 246)
PFOXO3a (Thr 32)
PERK1/2
PP70S6K (Thr 389)
PS6K (Ser 235/236)
Cyclin D1

control variables

FOXO3a
PRAS40
GSK3b
ERK1/2
P70S6K
β-actin
Tubulin

positive controls

PDK1 inhibitor GSK2334470

Annotations

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