Analytical Methods for Berberine and Metabolites

A liquid chromatography instrument coupled to an ion trap time-of-flight mass spectrometer (LC/MSⁿ-IT-TOF) from the Shimadzu Corporation (Kyoto, Japan) was used to identify the chemical structures of BBR and its metabolites. Liquid chromatography with tandem mass spectrometry (LC-MS/MS 8050, Shimadzu Corporation, Kyoto, Japan) and gas chromatography with mass spectrometry (GC-MS QP2010, Shimadzu Cooperation, Kyoto, Japan) were used for the analysis and quantification of BBR and its metabolites in biological samples 14 (link), 17 (link), 20 (link).

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Wang Y., Tong Q., Shou J.W., Zhao Z.X., Li X.Y., Zhang X.F., Ma S.R., He C.Y., Lin Y., Wen B.Y., Guo F., Fu J, & Jiang J.D. (2017). Gut Microbiota-Mediated Personalized Treatment of Hyperlipidemia Using Berberine. Theranostics, 7(9), 2443-2451.

Publication 2017

LC/MSn-IT-TOF LC-MS/MS 8050 GC-MS QP2010

Biological Gc ms Liquid chromatography Ms ms

Corresponding Organization :

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College, First Hospital of Jilin University, Jilin University

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Variable analysis

independent variables

Liquid chromatography instrument coupled to an ion trap time-of-flight mass spectrometer (LC/MS^n-IT-TOF) from the Shimadzu Corporation (Kyoto, Japan)
Liquid chromatography with tandem mass spectrometry (LC-MS/MS 8050, Shimadzu Corporation, Kyoto, Japan)
Gas chromatography with mass spectrometry (GC-MS QP2010, Shimadzu Cooperation, Kyoto, Japan)

dependent variables

Identification of the chemical structures of BBR and its metabolites
Analysis and quantification of BBR and its metabolites in biological samples

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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