Evaluating Glucose, Lipid, and Insulin Metabolism

Blood glucose levels were measured by glucose oxidase assay (GLU GOD, Erba-Lachema, Czech Republic) using tail vein blood drawn into 5% trichloracetic acid and promptly centrifuged. Serum TAG concentrations were quantified using standard enzymatic methods (TG L 250 S, Erba-Lachema, Czech Republic). Non-esterified fatty acid levels (NEFA) were determined using an acyl-CoA oxidase-based colorimetric kit (Roche Diagnostics GmbH, Germany). Serum insulin concentrations were evaluated using a rat insulin enzyme-linked immunosorbent assay kit (Mercodia, Sweden). Irisin serum concentrations were measured using an irisin enzyme-linked immunosorbent assay kit (Irisin ELISA, BioVendor, Czech Republic). The insulin sensitivity index (ISI) was calculated from fasting insulin (FI) and fasting glucose (FG) as described previously [37 (link)]. ISI = 1/(FG*FI), where FG is expressed as mg/dl and FI as mIU/l.

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Škop V., Malínská H., Trnovská J., Hüttl M., Cahová M., Blachnio-Zabielska A., Baranowski M., Burian M., Oliyarnyk O, & Kazdová L. (2015). Positive Effects of Voluntary Running on Metabolic Syndrome-Related Disorders in Non-Obese Hereditary Hypertriacylglycerolemic Rats. PLoS ONE, 10(4), e0122768.

Publication 2015

acyl-CoA oxidase-based colorimetric kit rat insulin enzyme-linked immunosorbent assay kit Irisin ELISA

Acyl coa oxidase Assay Blood Blood glucose Colorimetric Diagnostics Elisa Enzymatic Erba Esterified fatty acid Glucose Glucose oxidase Insulin Insulin sensitivity Serum Tail Trichloracetic acid Vein

Corresponding Organization : Medical University of Białystok

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Blood glucose levels
Serum triglyceride (TAG) concentrations
Non-esterified fatty acid (NEFA) levels
Serum insulin concentrations
Irisin serum concentrations
Insulin sensitivity index (ISI)

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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