The following markers were used for live imaging: endo-DE-cadherin:GFP (Huang et al., 2009 (link)), ubi-DE-cadherin:GFP (Oda and Tsukita, 2001 (link)), UAS-DE-cadherin:GFP (gift of N. Gorfinkiel, Consejo Superior de Investigaciones Cientificas–Universidad Autónoma de Madrid, Madrid, Spain), UAS-p120-catenin:GFP (Bloomington Drosophila Stock Center; Myster et al., 2003 (link)), UAS-actin:RFP (Simões et al., 2006 (link); gift of N. Gorfinkiel), sqh-GFP:moesin (Kiehart et al., 2000 (link)), UAS-mCherry:moesin (Millard and Martin, 2008 (link)), sqh-sqh:GFP (Royou et al., 2004 (link)), sqh-sqh:mCherry (Martin et al., 2009 (link)), sqh-GFP:rokK116A (Simões et al., 2010 (link)), UAS-GFP:clc (Chang et al., 2002 (link)), 20XUAS-shits1:GFP (Pfeiffer et al., 2012 (link)), UAS-Apoliner (Bloomington Drosophila Stock Center; Bardet et al., 2008 (link)), UAS-GFP (gift of U. Tepass, University of Toronto, Toronto, Canada), 10XUAS-myr:GFP (Bloomington Drosophila Stock Center; Pfeiffer et al., 2010 (link)), UAS-GCaMP3 (Bloomington Drosophila Stock Center; Tian et al., 2009 (link)), and UAS-arf6:GFP (this study). daughterless-Gal4 or tubulin-Gal4 (Bloomington Drosophila Stock Center) were used to drive ubiquitous expression of all UAS transgenes. tubulin-Gal4 was used for all overexpression experiments. yellow white flies were used for controls.
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Live Imaging of Drosophila Cell Junctions and Dynamics
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Variable analysis
independent variables
- Daughterless-Gal4
- Tubulin-Gal4
- Expression of fluorescently labeled markers: endo-DE-cadherin:GFP, ubi-DE-cadherin:GFP, UAS-DE-cadherin:GFP, UAS-p120-catenin:GFP, UAS-actin:RFP, sqh-GFP:moesin, UAS-mCherry:moesin, sqh-sqh:GFP, sqh-sqh:mCherry, sqh-GFP:rok^K116A, UAS-GFP:clc, 20XUAS-shi^ts1:GFP, UAS-Apoliner, UAS-GFP, 10XUAS-myr:GFP, UAS-GCaMP3, UAS-arf6:GFP
- Yellow white flies
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