An associated biochemical kit (Cat No: TAC-96A, ZellBioGmbH-Germany) was purchased. The reaction of this kit is based on the oxidation colorimetric resuscitation. This kit contained 1 reagent ready to use, buffer X 200, dye powder, reaction suspension solution, standard, and a microplate of 96 wells. The sensitivity of the kit was equal to 0.2 mm, and final absorbance was read at 540 nm [6 (link)].
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