The pH and temperature were monitored monthly using a pH meter (Sartorius PB-10) and a thermometer separately. The different organic loading rates (OLRs) were achieved by various chemical oxygen demand (COD) concentrations at a fixed hydraulic retention time (HRT). The COD, total suspended solids (TSS), and volatile suspended solids (VSS) were measured according to standard methods (Rice et al., 2012 ). Biogas production was monthly monitored and the CH4 percentage was determined by Gas Chromatography (SRI 8610 C). The ammonium nitrogen and total nitrogen concentration were determined by the SmartChem Discrete Auto Analyzer (Smartchem200, AMS-Westco, Italy) and Shimadzu TOC Analyzer (TOC-Vcsh; Shimadzu), respectively.
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