Antibody Panel for Cellular Signaling Analysis

The following primary antibodies were used: mouse anti-p-H2AX Ser139 (sc-517348, Santa Cruz Biotechnology; JBW301; immunofluorescence, IF, and flow cytometry FC), rabbit anti-53BP (A300-272A, Bethyl Laboratories; IF), mouse anti-involucrin (SY3; lab made, Hudson et al., 1992; IF and FC), mouse anti-K13 (Novus Biologicals, Littleton, CO; FC and IF), mouse anti-K16 (sc-53255, Santa Cruz Biotechnology; IF and FC), mouse anti-p21CIP (CP74, Sigma-Aldrich; WB), mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; sc-47724; Santa Cruz Biotechnology; WB), mouse anti-α-tubulin (B-5-1-2, Santa Cruz Biotechnology, sc-23948), mouse anti-wee1 (B11, Santa Cruz Biotechnology, sc-5285, WB), rabbit anti-Cyclin-A (H432, Santa Cruz Biotechnology, sc-751; WB and IF), mouse anti-Cyclin-B (Santa Cruz Biotechnology, sc-245; WB and IF), rabbit anti-p-histone 3 (Santa Cruz Biotechnology, sc-8656-R; IF), mouse anti-CDK1-p34 (A17.1.1, Millipore, MAB8878; WB).
The following secondary antibodies from Jackson ImmunoResearch were used: Alexa Fluor® 488-conjugated goat anti-rabbit or anti-mouse IgG antibodies (115-547-003; FC and IF); Alexa Fluor® 594-conjugated goat anti-rabbit or anti-mouse IgG antibodies (115-517-003; IF). Other secondary antibodies used were: DyLightTM 800-conjugated goat anti-rabbit or anti-mouse IgG antibodies (ThermoFisher, WB) DyLightTM 488-conjugated goat anti-mouse IgG antibodies (35503, ThermoFisher; IF and FC) HRP conjugated goat anti-rabbit or anti-mouse IgG antibodies (Bio-Rad, WB).

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Juan L.S., Freije A., Sanz-Gómez N., Jiménez-Matías B., Pleguezuelos-Manzano C., Sanz J.R., de Diego E., Naranjo S., Clevers H, & Gandarillas A. (2023). DNA damage triggers squamous metaplasia in human lung and mammary cells via mitotic checkpoints. Cell Death Discovery, 9, 21.

Publication 2023

Alexa 594 Alexa fluor 488 Anti igg Antibodies Biologicals Cdk1 Cyclin a Cyclin b Flow cytometry Gapdh Glyceraldehyde 3 phosphate dehydrogenase Goat Histone Immunofluorescence Involucrin Mouse Novus Rabbit α tubulin

Corresponding Organization :

Other organizations : Instituto de Investigación Marqués de Valdecilla, Royal Netherlands Academy of Arts and Sciences, Oncode Institute, University Medical Center Utrecht

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

P-H2AX Ser139
Involucrin
P21CIP
GAPDH
α-tubulin
Cyclin-A
Cyclin-B
P-histone 3
CDK1-p34

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

Annotations

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