Culture and Isolation of CD34+ Progenitors

C/EBPA^N/C-8 cells were cultured at the concentration of 1 × 10⁶ cell/mL in StemSpanTM Serum-Free Expansion Medium II (SFEMII) plus 10% StemSpanTM CD34+ Expansion Supplement and 175 nM UM171 (STEMCELL Technologies). All other patient cells were grown in SFEMII supplemented with 100 ng/mL thrombopoietin, 10 ng/mL FMS-like tyrosine kinase 3 ligand, 750 nM Stem Regenin 1 (SRI), 10 ng/mL Interleukin 3, 10 ng/mL human granulocyte/macrophage colony stimulating factor, 150 ng/mL Stem Cell Factor (all Peprotech) and UM729 (STEMCELL Technologies). Patient CD34+ or CD117+ progenitors were isolated with CD34 MicroBead Kit, human or CD117 MicroBead Kit, human (Miltenyi-Biotech) as described in [4 (link)].