Lipid and Hormone Quantification

Serum concentrations of total triglyceride and cholesterol were directly determined by commercial assay kits based on glycerol phosphate oxidase-p-aminophenazone method. According to the instruction, the low-density lipoprotein cholesterol level was determined by commercial assay kits. Enzyme linked immunosorbent assay kits was applied to quantify concentrations of very low-density lipoprotein cholesterol, growth hormone, insulin, and leptin and insulin-like growth factors-1. All kits were supplied by Nanjing Jiancheng Bioengineering Institute (Nanjing, China).