Bacterial culture conditions and total RNA extracts were performed as the RNA-seq analysis. RNase-free DNase I was used to remove genomic DNA, and 1 μg RNA was used to generate cDNA using Hiscript III RT Super mix (Vazyme, Nanjing, China). The specific primers used for qRT-PCR are listed in Table S2 . The reaction mixtures were run on an ABI PRISM 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China). Transcript levels of the indicated genes were normalized to gyrB using the 2−ΔΔCt method [51 (link)]. Three independent experiments were performed.
Free full text: Click here
