An isotonic solution of MnCl2·4H2O (203734; Sigma-Aldrich) was prepared at a concentration of 100 mM in 100 mM bicine solution, as previously described (24 (link)). Then, the MnCl2 solution was diluted to 50 mM with saline, and the pH was adjusted to 7.4.
We intraperitoneally administered a 50 mM solution of MnCl2 immediately after the initial MRI acquisition, twice at 1-h intervals, to get a total dose of 66 mg/kg. MEMRI acquisition was performed 24 h after the first administration of MnCl2 based on the kinetics of intracerebral Mn2+ concentration (11 (link)). The imaging area was adjusted to the same position as in the initial MRI to facilitate comparison of T1 values at the same ROI.
We intraperitoneally administered a 50 mM solution of MnCl2 immediately after the initial MRI acquisition, twice at 1-h intervals, to get a total dose of 66 mg/kg. MEMRI acquisition was performed 24 h after the first administration of MnCl2 based on the kinetics of intracerebral Mn2+ concentration (11 (link)). The imaging area was adjusted to the same position as in the initial MRI to facilitate comparison of T1 values at the same ROI.
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